Petaka Advantages
Petaka Advantages
Oxygen Concentration
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- Category: pet_advantages
- Published on Thursday, 31 March 2011 07:58
- Written by Super User
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Petaka provides the cells with a gradually decreasing oxygen concentration in the media, within the physiological limits of living tissues (Graph 1).
Cell culture may begin with a concentration equivalent to that of arterial blood (OP 75 mmHg), high enough to promote exponential cell growth. The concentration is then progressively reduced to that of embryonic development (OP 15 mmHg), avoiding cell damage and facilitating cell differentiation.
Cultured cells need oxygen, but not in excess. When cells are cultured in media in classic cell culture devices, such as flasks and petri dishes, excessive oxygen is provided. In these devices, the media surface forms an interface with the atmosphere, which contains about 20% oxygen; through that interface, the oxygen in the air dissolves in the media up to the limit of its solubility in water, depending on cell culture temperature, atmospheric pressure and the degree of salinity of the media. This results in oxygen concentrations that are too high for normal cells, which naturally live in the lower oxygen environment found in living bodies.
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