Time Savings

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Category: pet_advantages

Published on Thursday, 31 March 2011 08:23

Written by Super User

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Inside the Petaka itself, one is able to perform several protocol steps without removing them from the device. This means that by not having to transfer cells to several other instruments or devices, one is able to cut out several steps. Examples of such applications are cell washing, cell & supernatant separation, enzyme-free detachment & harvesting, DNA & RNA extraction, cell pelleting, cell storage and pre-preparation of ready to host Petakas.

ComparativeAnalysis-PetakaETvsAUTOFLASK .pdf

ComparativeAnalysis-PetakaETvsOPTICELL .pdf

ComparativeAnalysis-PetakaETvsROBOFLASK .pdf

ComparativeAnalysis-PetakaETvsT75FLASK .pdf

ComparativeAnalysis-PetakaG3vsT75FLASK .pdf

Petaka Advantages .pdf

Cell Transport

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Category: pet_advantages

Published on Thursday, 31 March 2011 08:09

Written by Super User

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With the Petaka you can transport live cells for long distances at normal ambient temperatures. Petaka does not need dry ice or refrigeration to transport cells.

Its slim shape permits easy packaging and protection. Especially designed mailers are ideal for shipping one or two units.

Cells inside Petaka, at normal temperatures, remain in a dormant state for days, even weeks. Viability decreases progressively depending on the cell type. CHO-K1 cells, for example, can travel for 3 to 4 weeks maintaining 75% viability. Some tumor cells can travel for a month or more.

Petaka Advantages .pdf

Safe Handling

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Category: pet_advantages

Published on Thursday, 31 March 2011 08:10

Written by Super User

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Petaka's auto-sealing port, a pre-slit, 1 mm thick, silicon diaphragm, allows easy penetration with plastic and metal tips up to 1 mm external diameter.

Petaka's 0.2 mm Filtered Vent: The internal chamber communicates with the external atmosphere through an in/out vent closed with a 0.2 µm pore filter.
By avoiding caps and lids, the duo vent-port simplifies the repetitive operations of cell culture and handling, and minimizes routine operations

When the user initiates the injection procedure, the fluids flow through the tip into an atrium communicating with the culture chamber through a protective mini-tube. Therefore, during injection, the interior of the cell culture chamber is never exposed to the external atmosphere, through any aperture. In addition the mini-tube breaks any back pressure, avoiding leaks through the port slit.

The diaphragm surface is accessible for swabbing with ethanol, and can be exposed to a gas flame in order to guarantee surface sterility.

The port assembly allows safe and quick in/out maneuvers with reliable seal, greatly minimizing contamination risk.

Petaka Advantages .pdf

Cell Dormancy

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Category: pet_advantages

Published on Thursday, 31 March 2011 08:07

Written by Super User

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Using regular flasks, cells should be subcultured as soon as possible when they reach a confluent monolayer state or saturate the carriers.

With Petaka, grown cell cultures can be maintained alive at room temperature, without dehydration risk, for long time periods.This facilitates cell culture management and maintenance routines; and provides additional advantages for cell biology and toxicology research (see "Petaka Quick Protocols" for conditions and methods, such as protocols 4 and 8 - "Keeping Cells in In-Vitro Dormancy").

Cell conservation in dormant state is favored by transferring cultures from 37° C to 22° C when cells are in a slow progressing cell cycle. This is achieved in an 80% confluent state.
Under low temperature and low pH, cells reduce DNA replication practically to zero, and remain in a suspended animation-like state (a certain accumulation of cells in G2 phase could be expected).The duration of the dormancy period is dependent on the cell type. At a minimum, 7 days survival is common for most cells, while others could live for more than 200 days.

Petaka Advantages .pdf